cuvette bio rad Search Results


electroporation cuvette  (Bio-Rad)
96
Bio-Rad electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electroporation cuvette/product/Bio-Rad
Average 96 stars, based on 1 article reviews
electroporation cuvette - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
cuvette  (Bio-Rad)
96
Bio-Rad cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cuvette/product/Bio-Rad
Average 96 stars, based on 1 article reviews
cuvette - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
biorad cuvettes  (Bio-Rad)
96
Bio-Rad biorad cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Biorad Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biorad cuvettes/product/Bio-Rad
Average 96 stars, based on 1 article reviews
biorad cuvettes - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
truview disposable cuvettes  (Bio-Rad)
93
Bio-Rad truview disposable cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Truview Disposable Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/truview disposable cuvettes/product/Bio-Rad
Average 93 stars, based on 1 article reviews
truview disposable cuvettes - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
gene pulser electroporation cuvette  (Bio-Rad)
96
Bio-Rad gene pulser electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene pulser electroporation cuvette/product/Bio-Rad
Average 96 stars, based on 1 article reviews
gene pulser electroporation cuvette - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
bio rad gene pulser electroporation system  (Bio-Rad)
96
Bio-Rad bio rad gene pulser electroporation system
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Bio Rad Gene Pulser Electroporation System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bio rad gene pulser electroporation system/product/Bio-Rad
Average 96 stars, based on 1 article reviews
bio rad gene pulser electroporation system - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
gene pulser cuvettes  (Bio-Rad)
95
Bio-Rad gene pulser cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene pulser cuvettes/product/Bio-Rad
Average 95 stars, based on 1 article reviews
gene pulser cuvettes - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
disposable polystyrene cuvettes  (Bio-Rad)
93
Bio-Rad disposable polystyrene cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Disposable Polystyrene Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/disposable polystyrene cuvettes/product/Bio-Rad
Average 93 stars, based on 1 article reviews
disposable polystyrene cuvettes - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
semi microvolume disposable cuvettes  (Bio-Rad)
93
Bio-Rad semi microvolume disposable cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Semi Microvolume Disposable Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/semi microvolume disposable cuvettes/product/Bio-Rad
Average 93 stars, based on 1 article reviews
semi microvolume disposable cuvettes - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
cuvette holder  (Bio-Rad)
90
Bio-Rad cuvette holder
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Cuvette Holder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cuvette holder/product/Bio-Rad
Average 90 stars, based on 1 article reviews
cuvette holder - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
ice cold electroporation cuvette  (Bio-Rad)
96
Bio-Rad ice cold electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Ice Cold Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ice cold electroporation cuvette/product/Bio-Rad
Average 96 stars, based on 1 article reviews
ice cold electroporation cuvette - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
sali 5495p tranlucent hygb expression vector dna  (Bio-Rad)
93
Bio-Rad sali 5495p tranlucent hygb expression vector dna
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Sali 5495p Tranlucent Hygb Expression Vector Dna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sali 5495p tranlucent hygb expression vector dna/product/Bio-Rad
Average 93 stars, based on 1 article reviews
sali 5495p tranlucent hygb expression vector dna - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier

Image Search Results


(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe electroporation (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .

Journal: bioRxiv

Article Title: Synaptopodin enables directional mechanoadaptation of integrin-based adhesions

doi: 10.64898/2026.01.25.701551

Figure Lengend Snippet: (a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe electroporation (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .

Article Snippet: Cells were transferred to a 4 mm electroporation cuvette (Bio-Rad, 1652088) and electroporated using a Gene Pulser Xcell system (Bio-Rad) with the following parameters: 140 V, square wave pulse, 25 ms duration.

Techniques: Immunofluorescence, Transfection, Electroporation, Staining, Expressing, Comparison, Control, Imaging